Preparation of Dehydrated Media

1.    APPARATUS

1. Autoclave
2. pH – meter
3. Hot air Oven
4. Analytical Balance
5. Test tubes
6. Media bottles 
7. Laminar Air Flow Unit

2.    REAGENTS  AND  MEDIAS

1. Brilliant Green Agar
2. Bismuth sulphate agar
3. Baird Parker Agar
4. Brilliant green bile broth
5. Cetrimide agar
6. Deoxycholate citrate agar
7. EMB agar
8. Fluid Lactose broth
9. Macconkey agar
10. Macconkey broth
11. Mannitol Salt agar
12. Nutrient agar
13. Nutrient broth
14. Pseudomonas agar, Fluorescene
15. Pseudomonas agar, Pyocyanin
16. Plate count agar
17. Potato Dextrose agar
18. R 2 A agar
19. Soyabean casein digest agar
20. Soyabean casein digest medium
21. Fluid selenite cystiene medium
22. Sabouraud dextrose agar
23. Sabouraud chloramphenical agar
24. Tetrathionate broth
25. TSI Agar
26. V-J agar
27. Xylose –Lysine Deoxycholate agar

All the above liquid media should be incubated for 48 hours at 32.5 ± 2.5 ⁰C before use. Any contaminated media should be discarded. 

 

1. PROCEDURE    
   1. Brilliant green agar

Make	Code
HIMEDIA	B

   PREPARATION PROCEDURE

1. Weigh 58.09 g of media in a butter paper using a clean weighing balance.
2. Transfer the weighed media into clean bottle / beaker  containing 1000 ml of distilled water/ Purified water.
3. Adjust the final pH (at 25⁰C) 6.9 ± 0.2.
4. Heat to dissolve the medium completely by keeping the beaker / bottle on the hot plate.
5. Sterilize the media bottle in an autoclave at 121–124⁰C for 30 minutes.
6. Avoid over heating of the media. 
7. After sterilization cool the media to 40 – 50⁰C.
8. Dispense about 15 to 20 ml of the media into Petri plates under Laminar Airflow. Allow it to solidify.

Purpose

1. Brilliant green agar is recommended for selective identification of Salmonella species.

 Storage condition

1.  Hygroscopic. Store in a cool dry place below 30⁰C away from bright light.

DISPOSAL OF MEDIA

1. The completed / used media/ unusal preparations of this media and derivates of the media there of are to be disposed off by Autoclaving.
2. Bismuth sulphite agar

Make	Code
HIMEDIA	C

 

            PREPARATION PROCEDURE

1. Weigh 52.32 g of media in a butter paper using a clean weighing balance.
2. Transfer the weighed media into clean Media bottle containing 1000 ml of distilled water/ Purified water.
3. Adjust the final pH (at 25⁰C) 7.6 ± 0.2.
4. Heat to dissolve the medium completely by keeping bottle on the hot plate.
5. Sterilize the media bottle in an autoclave at 121–124⁰C for 30 minutes.
6. Avoid over heating of the media. 
7. After sterilization cool the media to 40 – 50⁰C.
8. Dispense about 15 to 20 ml of the media into Petri plates under Laminar Airflow. Allow it to solidify.

Purpose

1. Bismuth sulphite agar is recommended for selective identification of Salmonella species.

Storage condition

1.  Hygroscopic. Store in a cool dry place below 30⁰C away from bright light.

DISPOSAL OF MEDIA

1. The completed / used media/ unusual preparations of this media and derivates of the media there of are to be disposed off by Autoclaving.
2. Baired parker agar

Make	Code
HIMEDIA	Q

  PREPARATION PROCEDURE

1. Weigh 63 g of media in a butter paper using a clean weighing balance.
2. Transfer the weighed media into clean Media bottle containing 950 ml of distilled water/ Purified water.
3. Adjust the final pH (at 25⁰C) 7.0 ± 0.2.
4. Heat to dissolve the medium completely by keeping bottle on the hot plate.
5. Sterilize the media bottle in an Autoclave at 121–124⁰C for 30 minutes.
6. After sterilization cool the media to 40 – 50⁰C.
7. Aseptically add 50 ml of Conc. Egg yolk emulsion (FD045) and 3 ml of    3.5 % Sterile potassium tellurite or 50 ml of Egg yolk tellurite Emultion.
8. Mix well and Dispense about 15 to 20 ml of the media into Petri plates under Laminar Airflow. Allow it to solidify.

Purpose

1. Baired parker agar is recommended for selective identification of Staphylococci.

 Storage condition

1.  Hygroscopic. Store in a cool dry place below 30⁰C away from bright  light.

DISPOSAL OF MEDIA

1. The completed / used media/ unusual preparations of this media and derivates of the media there of are to be disposed off by Autoclaving.
2. Brilliant green bile broth

Make	Code
HIMEDIA	X

            PREPARATION PROCEDURE

1. Weigh 40.0 g of media in a butter paper using a clean weighing balance.
2. Transfer the weighed media into clean Media bottle containing 1000 ml of distilled water/ Purified water.
3. Adjust the final pH (at 25⁰C) 7.2 ± 0.2.
4. Heat to dissolve the medium completely by keeping bottle on the hot plate.
5. Dispense the medium into test tubes as per requirement and Sterilize by Autoclaving.
6. After sterilization cool the media to room temperature.

Purpose

1. Brilliant green bile broth is recommended for selective enrichment of coliforms.

 Storage condition

1.  Hygroscopic. Store in a cool dry place below 30⁰C away from bright  light.

DISPOSAL OF MEDIA

1. The completed / used media/ unusual preparations of this media and derivates of the media there of are to be disposed off by Autoclaving.
2. Cetrimide agar

Make	Code
HIMEDIA	D

            PREPARATION PROCEDURE

1. Weigh 45.3 g of media in a butter paper using a clean weighing balance.
2. Transfer the weighed media into clean Media bottle containing 1000 ml of distilled water/ Purified water.
3. Adjust the final pH (at 25⁰C) 7.2 ± 0.2.
4. Heat to dissolve the medium completely by keeping bottle on the hot plate.
5. Sterilize the media bottle in an Autoclave at 121–124⁰C for 30 minutes.
6. After sterilization cool the media to 40 – 50⁰C.
7. Dispense about 15 to 20 ml of the media into Petri plates under Laminar Airflow. Allow it to solidify.

Purpose

1. Cetrimide agar is recommended for selective identification of Pseudomonas.

 Storage condition

1.  Hygroscopic. Store in a cool dry place below 30⁰C away from bright light.

DISPOSAL OF MEDIA

1. The completed / used media/ unusual preparations of this media and derivates of the media there of are to be disposed off by Autoclaving.
2. Deoxycholate citrate agar

Make	Code
HIMEDIA	K

            PREPARATION PROCEDURE

1. Weigh 70.52 g of media in a butter paper using a clean weighing balance.
2. Transfer the weighed media into clean Media bottle containing 1000 ml of distilled water/ Purified water.
3. Adjust the final pH (at 25⁰C) 7.5 ± 0.2.
4. Heat to dissolve the medium completely by keeping bottle on the hot plate.
5. Boil to dissolve the medium. Do not Autoclave and avoid over heating.
6. After boiling cool the media to 40 – 50⁰C.
7. Dispense about 15 to 20 ml of the media into Petri plates under Laminar Airflow. Allow it to solidify.

Purpose

1. Deoxycholate citrate agar is recommended for selective identification of Salmonella species.

 Storage condition

1.  Hygroscopic. Store in a cool dry place below 25⁰C away from bright light.

DISPOSAL OF MEDIA

1. The completed / used media/ unusual preparations of this media and derivates of the media there of are to be disposed off by Autoclaving.
2. EMB  agar

Make	Code
HIMEDIA	J

            PREPARATION PROCEDURE

1. Weigh 36 g of media in a butter paper using a clean weighing balance.
2. Transfer the weighed media into clean Media bottle containing 1000 ml of distilled water/ Purified water.
3. Adjust the final pH (at 25⁰C) 7.2 ± 0.2.
4. Heat to dissolve the medium completely by keeping bottle on the hot plate.
5. Sterilize the media bottle in an autoclave at 121–124⁰C for 30 minutes. Avoid over Heating.
6. After sterilization cool the media to 40 – 50⁰C.
7. Dispense about 15 to 20 ml of the media into Petri plates under Laminar Airflow. Allow it to solidify.

Purpose

1. EMB agar is recommended for selective identification of Escherichia coli.

Storage condition

1.  Hygroscopic. Store in a cool dry place below 30⁰C away from bright light.

DISPOSAL OF MEDIA

1. The completed / used media/ unusual preparations of this media and derivates of the media there of are to be disposed off by  Autoclaving.
2. Fluid Lactose broth

Make	Code
HIMEDIA	Y

            PREPARATION PROCEDURE

1. Weigh 13.0 g of media in a butter paper using a clean weighing balance.
2. Transfer the weighed media into clean Media bottle containing 1000 ml of distilled water/ Purified water.
3. Adjust the final pH (at 25⁰C) 6.9 ± 0.2.
4. Heat to dissolve the medium completely by keeping bottle on the hot plate.
5. Dispense the medium into test tubes as per requirement.
6. Sterilize the medium at 121 – 124⁰C for 30 minutes
7. After sterilization cool the media to room temperature.

Purpose

1. Fluid lactose broth is recommended as enrichment medium.

Storage condition

1.  Hygroscopic. Store in a cool dry place below 30⁰C away from bright  light.

DISPOSAL OF MEDIA

1. The completed / used media/ unusual preparations of this media and derivates of the media there of are to be disposed off by Autoclaving.
2. Macconkey agar

Make	Code
HIMEDIA	G

            PREPARATION PROCEDURE

1. Weigh 50 g of media in a butter paper using a clean weighing balance.
2. Transfer the weighed media into clean Media bottle containing 1000 ml of distilled water/ Purified water.
3. Adjust the final pH (at 25⁰C) 7.1 ± 0.2.
4. Heat to dissolve the medium completely by keeping bottle on the hot plate.
5. Sterilize the media bottle in an autoclave at 121–124⁰C for 30 minutes.
6. After sterilization cool the media to 40 – 50⁰C.
7. Dispense about 15 to 20 ml of the media into Petri plates under Laminar Airflow. Allow it to solidify.

Purpose

1. Macconkey agar is recommended for selective identification of Escherichia coli.

 Storage condition

1.  Hygroscopic. Store in a cool dry place below 30⁰C away from bright  light.

DISPOSAL OF MEDIA

1. The completed / used media/ unusual preparations of this media and derivates of the media there of are to be disposed off by autoclaving.
2. Macconkey broth

Make	Code
HIMEDIA	H

PREPARATION PROCEDURE

1. Weigh 40.0 g of media in a butter paper using a clean weighing balance.
2. Transfer the weighed media into clean Media bottle containing 1000 ml of distilled water/ Purified water.
3. Adjust the final pH (at 25⁰C) 7.3 ± 0.2.
4. Heat to dissolve the medium completely by keeping bottle on the hot plate.
5. Dispense the medium into test tubes as per requirement.
6. Sterilize the medium at 121 – 124⁰C for 30 minutes
7. After sterilization cool the media to room temperature.

Purpose

1. Macconkey broth is recommended as selective enrichment medium for Escherichia coli.

 Storage condition

1.  Hygroscopic. Store in a cool dry place below 30⁰C away from bright light.

DISPOSAL OF MEDIA

1. The completed / used media/ unusual preparations of this media and derivates of the media there of are to be disposed off by Autoclaving.
2. Mannitol Salt agar

Make	Code
HIMEDIA	I

            PREPARATION PROCEDURE

1. Weigh 111 g of media in a butter paper using a clean weighing balance.
2. Transfer the weighed media into clean Media bottle containing 1000 ml of distilled water/ Purified water.
3. Adjust the final pH (at 25⁰C) 7.4 ± 0.2.
4. Heat to dissolve the medium completely by keeping bottle on the hot plate.
5. Sterilize the media bottle in an autoclave at 121–124⁰C for 30 minutes.
6. After sterilization cool the media to 40 – 50⁰C.
7. Dispense about 15 to 20 ml of the media into Petri plates under Laminar Airflow. Allow it to solidify.

Purpose

1. Mannitol Salt agar is recommended for selective identification of Staphylococcus aureus.

Storage condition

1.  Hygroscopic. Store in a cool dry place below 30⁰C away from bright  light.

DISPOSAL OF MEDIA

1. The completed / used media/ unusual preparations of this media and derivates of the media there of are to be disposed off by autoclaving.
2. Nutrient agar

Make	Code
HIMEDIA	U

            PREPARATION PROCEDURE

1. Weigh 28 g of media in a butter paper using a clean weighing balance.
2. Transfer the weighed media into clean Media bottle containing 1000 ml of distilled water/ Purified water.
3. Adjust the final pH (at 25⁰C) 7.4 ± 0.2.
4. Heat to dissolve the medium completely by keeping bottle on the hot plate.
5. Sterilize the media bottle in an Autoclave at 121–124⁰C for 30 minutes.
6. After sterilization cool the media to 40 – 50⁰C.
7. Dispense about 15 to 20 ml of the media into Petri plates under Laminar Airflow. Allow it to solidify.

Purpose

1. Nutrient agar is recommended cultivation wide variety of microorganisms.

 Storage condition

1.  Hygroscopic. Store in a cool dry place below 30⁰C away from bright  light.

DISPOSAL OF MEDIA

The completed / used media/ unusual preparations of this media and derivates of the media there of are to be disposed off by Autoclaving.

1. Nutrient broth

Make	Code
HIMEDIA	F

            PREPARATION PROCEDURE

1. Weigh 8.0 g of media in a butter paper using a clean weighing balance.
2. Transfer the weighed media into clean Media bottle containing 1000 ml of distilled water/ Purified water.
3. Adjust the final pH (at 25⁰C) 6.9 ± 0.2.
4. Heat to dissolve the medium completely by keeping bottle on the hot plate.
5. Dispense the medium into test tubes as per requirement.
6. Sterilize in water bath or free flowing steam for 10 minutes.
7. After sterilization cool the media to room temperature.

Purpose

1. Nutrient  broth is recommended as general purpose medium for the cultivation of microorganisms.

Storage condition

1.  Hygroscopic. Store in a cool dry place below 30⁰C away from bright  light.

DISPOSAL OF MEDIA

The completed / used media/ unusual preparations of this media and derivates of the media there of are to be disposed off by autoclaving.

1. Pseudomonas agar ( Fluorescien)

Make	Code
HIMEDIA	Z

            PREPARATION PROCEDURE

1. Weigh 38 g of media in a butter paper using a clean weighing balance.
2. Transfer the weighed media into clean Media bottle containing 1000 ml of distilled water/ Purified water.
3. Adjust the final pH (at 25⁰C) 7.0 ± 0.2.
4. Heat to dissolve the medium completely by keeping bottle on the hot plate.
5. Sterilize the media bottle in and  Autoclave at 121–124⁰C for 30 minutes.
6. After sterilization cool the media to 40 – 50⁰C.
7. Dispense about 15 to 20 ml of the media into Petri plates under Laminar Airflow. Allow it to solidify.

Purpose

1. Pseudomonas agar is recommended for selective identification of Pseudomonas.

Storage condition

1.  Hygroscopic. Store in a cool dry place below 30⁰C away from bright  light.

DISPOSAL OF MEDIA

1. The completed / used media/ unusual preparations of this media and derivates of the media there of are to be disposed off by  Autoclaving.
2. Pseudomonas agar, pyocyanin

Make	Code
HIMEDIA	AA

            PREPARATION PROCEDURE

1. Weigh 46.4 g of media in a butter paper using a clean weighing balance.
2. Transfer the weighed media into clean Media bottle containing 1000 ml of distilled water/ Purified water.
3. Adjust the final pH (at 25⁰C) 7.0 ± 0.2.
4. Heat to dissolve the medium completely by keeping bottle on the hot plate.
5. Sterilize the media bottle in an Autoclave at 121–124⁰C for 30 minutes.
6. After sterilization cool the media to 40 – 50⁰C.
7. Dispense about 15 to 20 ml of the media into Petri plates under Laminar Airflow. Allow it to solidify.

Purpose

1. Pseudomonas agar is recommended for selective identification of Pseudomonas.

 Storage condition

1.  Hygroscopic. Store in a cool dry place below 30⁰C away from bright  light.

DISPOSAL OF MEDIA

1. The completed / used media/ unusual preparations of this media and derivates of the media there of are to be disposed off by Autoclaving.
2. Plate count agar

Make	Code
HIMEDIA	S

            PREPARATION PROCEDURE

1. Weigh 23.5 g of media in a butter paper using a clean weighing balance.
2. Transfer the weighed media into clean Media bottle containing 1000 ml of distilled water/ Purified water.
3. Adjust the final pH (at 25⁰C) 7.0 ± 0.2.
4. Heat to dissolve the medium completely by keeping bottle on the hot plate.
5. Sterilize the media bottle in an Autoclave at 121–124⁰C for 30 minutes.
6. After sterilization cool the media to 40 – 50⁰C .
7. Dispense about 15 to 20 ml of the media into Petri plates under Laminar Airflow. Allow it to solidify.

Purpose

1. plate count agar is recommended for the enumeration of bacteria in water.

Storage condition

1.  Hygroscopic. Store in a cool dry place below 30⁰C away from bright  light.

DISPOSAL OF MEDIA

1. The completed / used media/ unusual preparations of this media and derivates of the media there of are to be disposed off by Autoclaving.

 

1. Potato Dextrose agar

Make	Code
HIMEDIA	BB

PREPARATION PROCEDURE

1. Weigh 41 g of media in a butter paper using a clean weighing balance.
2. Transfer the weighed media into clean Media bottle containing 1000 ml of distilled water/ Purified water.
3. Adjust the final pH (at 25⁰C) 5.6 ± 0.2.
4. Heat to dissolve the medium completely by keeping bottle on the hot plate.
5. Dispense as required and Sterilize the media bottle in an Autoclave at 121–124⁰C for 30 minutes.
6. After sterilization cool the media to 40 – 50⁰C.

Purpose

1. Potato dextrose agar is recommended as growth medium for Yeast and Moulds.

 Storage condition

1.  Hygroscopic. Store in a cool dry place below 30⁰C away from bright  light.

DISPOSAL OF MEDIA

1. The completed / used media/ unusual preparations of this media and derivates of the media there of are to be disposed off by  Autoclaving.
2. R 2 A agar

Make	Code
HIMEDIA	T

            PREPARATION PROCEDURE

1. Weigh 18.12 g of media in a butter paper using a clean weighing balance.
2. Transfer the weighed media into clean Media bottle containing 1000 ml of distilled water/ Purified water.
3. Adjust the final pH (at 25⁰C) 7.2 ± 0.2.
4. Heat to dissolve the medium completely by keeping bottle on the hot plate.
5. Dispense as required and Sterilize the media bottle in an Autoclave at 121–124⁰C for 30 minutes.
6. After sterilization cool the media to 40 – 50⁰C and distribute the media into petriplates.

Purpose

1. R 2A  agar is recommended as growth medium for bacteria.

 Storage condition

1.  Hygroscopic. Store in a cool dry place below 30⁰C away from bright  light.

DISPOSAL OF MEDIA

1. The completed / used media/ unusual preparations of this media and derivates of the media there of are to be disposed off by Autoclaving.
2. Soyabean casein digest agar

Make	Code
HIMEDIA	R

        PREPARATION PROCEDURE

1. Weigh 40 g of media in a butter paper using a clean weighing balance.
2. Transfer the weighed media into clean Media bottle containing 1000 ml of distilled water/ Purified water.
3. Adjust the final pH (at 25⁰C) 7.3 ± 0.2.
4. Heat to dissolve the medium completely by keeping bottle on the hot plate.
5. Dispense as required and Sterilize the media bottle in an Autoclave at 121–124⁰C for 30 minutes.
6. After sterilization cool the media to 40 – 50⁰C and distribute the medium into petriplates.

Purpose

1. Soyabean casein digest agar is recommended as growth medium for Bacteria.

 Storage condition

1.  Hygroscopic. Store in a cool dry place below 30⁰C away from bright  light.

DISPOSAL OF MEDIA

1. The completed / used media/ unusual preparations of this media and derivates of the media there of are to be disposed off by  Autoclaving.
2. Fluid selenite cystiene  medium

Make	Code
HIMEDIA	M

            PREPARATION PROCEDURE

1. Weigh 23.0 g of media in a butter paper using a clean weighing balance.
2. Transfer the weighed media into clean Media bottle containing 1000 ml of distilled water/ Purified water.
3. Adjust the final pH (at 25⁰C) 7.0 ± 0.2.
4. Heat to dissolve the medium completely by keeping bottle on the hot plate.
5. Dispense the medium into test tubes as per requirement.
6. Sterilize in water bath or free flowing steam for 10 minutes.
7. After sterilization cool the media to room temperature.

Purpose

1. fluid selenite cystiene broth is recommended as selective enrichment medium for Salmonella species.

 Storage condition

1.  Hygroscopic. Store in a cool dry place below 30⁰C away from bright  light.

DISPOSAL OF MEDIA

The completed / used media/ unusual preparations of this media and derivates of the media there of are to be disposed off by  Autoclaving.

1. Soyabean Casein digest  medium

Make	Code
HIMEDIA	R

            PREPARATION PROCEDURE

1. Weigh 30.0 g of media in a butter paper using a clean weighing balance.
2. Transfer the weighed media into clean Media bottle containing 1000 ml of distilled water/ Purified water.
3. Adjust the final pH (at 25⁰C) 7.3 ± 0.2.
4. Heat to dissolve the medium completely by keeping bottle on the hot plate.
5. Dispense the medium into test tubes as per requirement.
6. Sterilize the medium  Autoclave  at 121 – 124⁰C for 30 minutes.
7. After sterilization cool the media to room temperature.

Purpose

1. Soyabean casein digest medium is recommended as selective enrichment medium.

 Storage condition

1.  Hygroscopic. Store in a cool dry place below 30⁰C away from bright  light.

DISPOSAL OF MEDIA

1. The completed / used media/ unusual preparations of this media and derivates of the media there of are to be disposed off by Autoclaving.
2. Sabouraud dextrose agar

Make	Code
HIMEDIA	II

       PREPARATION PROCEDURE

1. Weigh 65 g of media in a butter paper using a clean weighing balance.
2. Transfer the weighed media into clean Media bottle containing 1000 ml of distilled water/ Purified water.
3. Adjust the final pH (at 25⁰C) 5.6 ± 0.2.
4. Heat to dissolve the medium completely by keeping bottle on the hot plate.
5. Dispense as required and Sterilize the media bottle in an Autoclave at 121–124⁰C for 30 minutes.
6. After sterilization cool the media to 40 – 50⁰C and distribute the medium into petriplates.

Purpose

1. Sabouraud dextrose agar is recommended as growth medium for yeast and Moulds.

 Storage condition

1.  Hygroscopic. Store in a cool dry place below 30⁰C away from bright  light.

DISPOSAL OF MEDIA

1. The completed / used media/ unusual preparations of this media and derivates of the media there of are to be disposed off by  Autoclaving.
2. Sabouraud chloramphenicol agar     

Make	Code
HIMEDIA	P

 PREPARATION PROCEDURE

1. Weigh 65 g of media in a butter paper using a clean weighing balance.
2. Transfer the weighed media into clean Media bottle containing 1000 ml of distilled water/ Purified water.
3. Adjust the final pH (at 25⁰C) 5.6 ± 0.2.
4. Heat to dissolve the medium completely by keeping bottle on the hot plate.
5. Dispense as required and Sterilize the media bottle in an Autoclave at 121–124⁰C for 30 minutes.
6. After sterilization cool the media to 40 – 50⁰C and distribute the medium into petriplates.

Purpose

1. Sabouraud chloramphenicol agar is recommended as growth medium for yeast and Moulds.

 Storage condition

1.  Hygroscopic. Store in a cool  place below 8⁰C.

DISPOSAL OF MEDIA

1. The completed / used media/ unusual preparations of this media and derivates of the media there of are to be disposed off by Autoclaving.
2. Tetrathionate broth

Make	Code
HIMEDIA	O

            PREPARATION PROCEDURE

1. Weigh 46.0 g of media in a butter paper using a clean weighing balance.
2. Transfer the weighed media into clean Media bottle containing 1000 ml of distilled water/ Purified water.
3. Adjust the final pH (at 25⁰C) 7.0 ± 0.2.
4. Heat to dissolve the medium completely by keeping bottle on the hot plate.
5. Dispense the medium into test tubes as per requirement.
6. Heat to boil. Do not over heat.
7. After boiling cool the media to room temperature.

Purpose

1. Tetrathionate broth is recommended as selective enrichment medium for Salmonella species.

Storage condition

1.  Hygroscopic. Store in a cool dry place below 30⁰C away from bright  light.

DISPOSAL OF MEDIA

1. The completed / used media/ unusual preparations of this media and derivates of the media there of are to be disposed off by  Autoclaving.
2. TSI agar

Make	Code
HIMEDIA	N

        PREPARATION PROCEDURE

1. Weigh 65 g of media in a butter paper using a clean weighing balance.
2. Transfer the weighed media into clean Media bottle containing 1000 ml of distilled water/ Purified water.
3. Adjust the final pH (at 25⁰C) 7.4 ± 0.2.
4. Heat to dissolve the medium completely by keeping bottle on the hot plate.
5. Dispense as required and Sterilize the media bottle in an Autoclave at  121-124⁰C for  30 minutes.

Purpose

1. TSI agar is recommended as confirmatory medium.

 Storage condition

1.  Hygroscopic. Store in a cool dry place below 30⁰C away from bright  light.

DISPOSAL OF MEDIA

1. The completed / used media/ unusual preparations of this media and derivates of the media there of are to be disposed off by Autoclaving.
2. V J agar

Make	Code
HIMEDIA	V

           PREPARATION PROCEDURE

1. Weigh 61 g of media in a butter paper using a clean weighing balance.
2. Transfer the weighed media into clean Media bottle containing 1000 ml of distilled water/ Purified water.
3. Adjust the final pH (at 25⁰C) 7.2 ± 0.2.
4. Heat to dissolve the medium completely by keeping bottle on the hot plate.
5. Dispense as required and Sterilize the media bottle in an Autoclave at 121–124⁰C for 30 minutes.
6. After sterilization cool add 20 ml of 1% sterile Potassium tellurite medium and  distribute the medium into petriplates.

Purpose

1. V J agar is recommended as selective identification medium for Staphylococcus aureus.

 Storage condition

1.  Hygroscopic. Store in a cool dry place below 25⁰C away from bright  light.

DISPOSAL OF MEDIA

1. The completed / used media/ unusual preparations of this media and derivates of the media there of are to be disposed off by Autoclaving.
2. XLD agar

Make	Code
HIMEDIA	W

       PREPARATION PROCEDURE

1. Weigh 56.68 g of media in a butter paper using a clean weighing balance.
2. Transfer the weighed media into clean Media bottle containing 1000 ml of distilled water/ Purified water.
3. Adjust the final pH (at 25⁰C) 7.4 ± 0.2.
4. Heat to dissolve the medium completely by keeping bottle on the hot plate.
5. Heat to boil with frequent agitation . Dispense as required and Sterilize the media bottle in an Autoclave at 121–124⁰C for 30 minutes.
6.  Do not over heat
7. After sterilization cool the media to 40 – 50⁰C and distribute the medium into petriplates.

Purpose

1. XLD agar is recommended as selective identification medium for Salmonella species.

 Storage condition

1.  Hygroscopic. Store in a cool dry place below 30⁰C away from bright  light.

DISPOSAL OF MEDIA

1. The completed / used media/ unusual preparations of this media and derivates of the media there of are to be disposed off by Autoclaving.

 

Dehydrated, general testing procedure, ph – meter, hot air oven

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