To lay down a procedure for disposal of microbial culture media.
1.0 OBJECTIVE :
To lay down a procedure for disposal of microbial culture media.
2.0 SCOPE :
This SOP is applicable to disposal of microbial culture media in Microbiology laboratory.
3.0 RESPONSIBILITY :
QC-Microbiologist
Head – Quality Control
4.0 PROCEDURE :
4.1 For culture media tubes/flasks/plates/pipettes:
4.1.1 Autoclave the media tubes/ flasks/plates/pipettes at 121ºC for 30 minutes at 15 lbs. pressure.
4.1.2 Allow the temperature to cool to 90ºC and collect the contents of the tubes/ flasks/plates/pipettes into the LDPE bag and send it to effluent treatment plant for further distillation.
4.1.3 Soak the empty, test tubes, pipettes , petri¬ plates for 1 hour in 2.5% Savlon solutions.
4.1.4 Wash thoroughly under running tap water. Rinse with purified water and dry.
4.3 For used pipettes:
4.3.1 Soak the used pipettes in 2.5% Savlon solution for minimum 1 hour.
4.3.2 Wash thoroughly with plenty of tap water & then in purified water and dry.
Microbial culture, microbiology
