1. APPARATUS
1.1 Autoclave
1.2 BOD - Incubator
1.3 Bacteriological Incubator
1.4 pH – meter
1.5 Hot air Oven
1.6 Laminar Air flow
1.7 Analytical Balance
1.8 Spirit Lamp / Bunsen Burner
1.9 Water Bath
1.10 Micropipette
1.11 Petri dishes
1.12 Test tubes
1.13 Media bottles
2. REAGENTS AND MEDIAS
3. Prepare the media as per Respective GTP.
4. PROCEDURE
4.1 As per USP Microbial limit are as follows
4.1.1 Total Microbial count. – Perform as per Respective GTP.
4.1.2 Specified Microorganisms
4.1.2.1 E .coli - Perform as per Respective GTP.
4.1.2.2 Salmonella - Perform as per Respective GTP.
4.1.2.3 Pseudomonas - Perform as per Respective GTP.
4.1.2.4 Staphyococcus aureus - Perform as per Respective GTP.
4.2 As per EP Microbial limit are as follows
4.2.1 Total Microbial count. – Perform as per Respective GTP.
4.2.2 Specified Microorganisms
4.2.2.1 E .coli - Perform as per Respective GTP.
4.2.2.2 Salmonella - Perform as per Respective GTP.
4.2.2.3 Pseudomonas - Perform as per Respective GTP.
4.2.2.4 Staphyococcus aureus - Perform as per Respective GTP.
Autoclave, general testing procedure, laminar air flow, bod - incubator