To lay down the procedure for operation and calibration of UV cabinet.
1.0 PURPOSE:
1.1 To lay down the procedure for operation and calibration of UV cabinet.
2.0 SCOPE:
2.1 This SOP is applicable for operation and calibration of UV cabinet in Quality Control Department.
3.0 RESPONSIBILITY:
3.1 Assistant – QC
4.0 ACCOUNTABILITY:
4.1 Executive / Asst. Manager – QC
5.0 PROCEDURE:
5.1 General cleaning:
5.1.1 Ensure that the power supply to the instrument is switched OFF before cleaning.
5.1.2 Clean the apparatus with a clean dry cloth every day.
5.1.3 Once in a week remove adhered dust by wet mopping using detergent solution. Afterwards wipe the surface with a clean dry cloth to remove traces of detergent.
5.1.4 Precautions have to be taken to clean the equipment immediately with dry cloth to remove the moisture.
5.2 Operating procedure:
5.2.1 Switch “ON” the main power supply.
5.2.2 Open the cabinet door, by pulling the white knob located on the front side of the cabinet.
5.2.3 Place the TLC plate or any material inside it and close the cabinet door.
5.2.4 Three switches are provided on the top left corner of the instrument.
5.2.5 Switch 1-for UV light at short wavelength (254 nm).
5.2.6 Switch 2-for UV light at long wavelength (365 nm).
5.2.7 Switch 3-for general light (white light).
5.2.8 Select the required light and switch to “ON”.
5.2.9 Observe the plate for visualization of spot from the observation-compartment (window) located on the top of cabinet.
5.2.10 Switch ‘OFF’ the lamp or the instrument when not required.
5.3 Precautions:
5.3.1 Do not stare at UV rays for a long time.
5.3.2 Do not expose hands or any part of the body to UV light.
5.4 Calibration:
5.4.1 The lamp calibration should be done as follows:
5.4.1.1 Prepare the solution A & B as given below:
5.4.1.1.1 Solution A (0.04% w/v): Dissolve 20.0 mg of sodium salicylate in 50.0 ml of methanol (95%).
5.4.1.1.2 Solution B (0.2%): Dissolve 20.0 mg of sodium salicylate in 10.0 ml of methanol (95%).
5.4.2 Apply to a plate coated with silica gel G, 5 ?l (spot diameter about 5 mm) of solution A [for lamps of maximum output at about 254 nm] and solution B [for lamps of maximum output at about 365 nm].
5.4.3 Examine the spots in a position normal to the radiation in the UV cabinet at the respective wavelengths as described in 5.4.1.2.
5.4.4 The lamp should be capable of revealing without doubt the above spots clearly.
5.4.5 Record the results in the calibration record for UV cabinet.
5.4.6 Update the ‘CALIBRATED’ label affixed on the instrument.
5.4.7 Report any discrepancy observed during calibration or operating the instrument to department head and notify the defect to technical officer/service engineer to rectify the defect. Affix ‘UNDER MAINTENANCE’ label on the instrument till it get rectified.
5.4.8 Frequency of calibration:
5.4.8.1 Once 3 months and after each maintenance job.
Uv cabinet, afterwards wipe, capable, observation, compartment
