Operation Cleaning and Calibration of Air Sampler

1.0    OBJECTIVE:

To lay down a procedure for the operation and cleaning of Air Sampler.

2.0    SCOPE: 

This procedure is applicable for the Air Sampler used for Environmental monitoring. 

3.0    RESPONSIBILITY: 

Microbiologist: To perform the operation as per SOP.

Head – Quality Control: To ensure the compliance of the SOP.

4.0    PROCEDURE: 

4.1    Operation: 

4.1.1    Clean the Air Sampler unit body with lint free cloth soaked in 70 %v/v IPA.

4.1.2    Press the ON/OFF switch (black button), provided at the bottom of the air-sampling unit once to switch on the unit. 

4.1.3    The main switch (black button) shall be pushed twice to switch on the unit, if the automatic switch off timer operated hen last used.

4.1.4    Each time the ON/OFF switch is pressed, a ten seconds visual display presentation will appear displaying the following information.

Press “YES” to switch on the MAS-100 and Serial number of Instrument is shown on the display.

4.1.5 After a few seconds the display will indicate remaining time until next Calibration.

         
4.1.6    Once the visual display is stable, it will show the required programmed volume of air. 

4.1.7    The selection of the volume is made by pressing the “YES” and “NO” button, when the program is in the relevant sub-menu.

4.1.8    To select other Volumes, press “YES”. Accept a volume with “NO” the menu changes in to the start menu. Aspiration set Volume is 1000liters/min. 

4.1.9    To change the User with “YES” and accept it with “NO”.

4.1.10    Press the enter button, the display will show the following sub-menus:

4.1.11    To modify “Delay” with “YES” and accept with pressing “NO”.

4.1.12    Date and Time are display.

4.1.13    To start the Instrument, press “YES”. To change some of the parameters to press “NO” and change desired parameters. 

4.1.14    During the running, the display shows the sampled volume and counts down the time until the total sampling volume is reached

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4.1.15    After sampling cycle, the message “ passed test completed”

4.1.16    At the end of the Sampling, the total volume sampled is displayed and the time shows 0.00 and the “RED” (STOP) LED IS on.

4.1.17    Switching off:  In this position the air sampler will automatically shut down after 30 minutes. In all other positions the air sampler Switches off automatically after 5 Minutes.

4.1.18    Sampling Failed: If “failed repeat test” is displayed the test was interrupted by pressing “NO” during sampling.

   
4.1.8     If the air flow was blocked, the air sampler display shows “air flow blocked”. And the red LED is blinking.

4.1.19    The air sampler is provided with a plate holder, which is adjustable to allow different brands of contact plates to be fitted. 

4.1.20    The sampling head can be sanitized by treating the inside and outside surfaces with 70%v/v IPA.

4.1.21    Ensure the head has no obstructed holes (foreign matter, agar etc.).

4.1.22    Hold up to light to visually inspect.

4.1.23    Disinfect the cover of the air sampler at the beginning of each sampling program, with 70%v/v IPA.

4.1.24    Remove the cover of the air sampler by unscrewing it, holding the edge of the cover. 

4.1.25    Avoid touching the inside and outside of the drilled area.

4.1.26    Insert a closed filled “contact plate” into the retaining slots and remove its lid. 

4.1.27    Replace the sampling head and start the aspiration cycle. 

4.1.28    At the end of the cycle unscrew the sampling head, replace the “contact plate lid” and remove the “contact plate”

4.1.29    Be sure each contact plate is identified with the appropriate sample data and incubated at the specified temperature for the specified time period.

4.1.30    Air sampler is also provided with a rechargeable battery. 

4.1.31    When the LOW BATTERY message is flashing, the air sampler shall be connected to the battery charger. 

4.1.26    For the charging purpose a charge port is given at the bottom of the air-sampling unit.

4.1.27    Air sampling unit is also provided with a remote control switch.

4.2    Cleaning:

4.2.1    Clean the outer body of the air-sampling unit with a lint free cloth soaked in 70%v/v IPA before carrying it for the environmental monitoring.

4.2.2    Sanitize the aspirating head with 70%v/v IPA before using it for the environmental monitoring.

4.3    Calibration Frequency: Once in a Year from external Party

Air sampler, cleaning, microbiology