To provide a procedure to operate and maintain the Altech make UV documentation system.
I. PURPOSE :
To provide a procedure to operate and maintain the Altech make UV documentation system.
II. SCOPE :
This procedure is applicable for the UV documentation system at used for TLC testing in the Quality Control department
III. RESPONSIBILITY :
It is the responsibility of all the personnel involved in the analysis using of the equipment.
IV. DOCUMENT REFERENCE :
SOPs : Core procedure: Calibration of Equipment
Forms : Calibration of UV cabinet
V. OPERATION :
1.0 PRELIMINARY CHECK :
1.1 Check that the instrument and surroundings are clean, if not, clean with a soft cloth duster.
2.0 BASIC OPERATION :
2.1 Before using ensure the validity of calibration.
2.2 Switch on the power.
2.3 Switch on the normal light and open the front door pulling towards the front side to
keep the TLC plates
2.4 Place the TLC plate in UV chamber horizontally on the base of the chamber.
2.5 Switch on the particular nm wave length light i.e. 254 nm or 365 nm light at which the TLC is to be viewed .
2.6 Do not expose to UV light while handling the UV cabinet, use always normal light only while performing the operations.
2.7 Click on the Imaging software icon in the Computer connected to the documentation system.
2.8 Adjust the settings to get a clear image .
2.9 Observe the picture of the TLC plate
3.0 Freese the picture and copy the image and paste in the word template created for it.
3.1 Take a print out of the image.
3.2 Switch of the power after the completion of the test.
VI. CALIBRATION : Calibration Frequency: First week of every month
Calibration of 254 nm lamp.
1. Weigh about 40 mg of sodium salicylate and transfer into 100 ml volumetric flask dissolve and dilute to 100 ml with ethanol.
2. Apply 5 ?L of the above solution on a TLC plate (glass or aluminum) with a diameter of about 5 mm.
3. Examine the spot in UV cabinet under 254 nm keeping the plate normal to the radiation.
4. The standard spot should be revealed without doubt i.e clearly. Calibration of 365 nm lamp.
1. Weigh about 200 mg of sodium salicylate transfer into 100 ml volumetric flask dissolve and dilute to 100 ml with ethanol.
2. Apply 5 ?L of the above solution on a TLC plate (glass or aluminum) with a diameter of about 5 mm.
3. Examine the spot in UV cabinet under 365 nm keeping the plate normal to the radiation.
4. The standard spot should be revealed without doubt i.e clearly.
5. If the instrument fails in calibration, proceed as per the Core Procedure, “Calibration of Equipment”.
Altech, uv cabinet, ethanol, sodium salicylate
