To lay down the procedure for entry, exit and gowning for Controlled area in Microbiology lab
1.0 OBJECTIVE
1.1 To lay down the procedure for entry, exit and gowning for Controlled area in Microbiology lab
2.0 SCOPE
2.1 This SOP covers the Entry, Exit and Gowning procedures for Microbiology lab controlled area in
3.0 RESPONSIBILITY
3.1 Microbiologist
4.0 ACCOUNTABILITY
4.1 Head - Quality Control
5.0 PROCEDURE
5.1 Entry Procedure with Gowning
5.1.1 Push the door to enter in to the first change room.
5.1.2 Remove the apron, cap and foot ware and keep them in respective cupboard.
5.1.3 Take a sterile gown from the Garment storage cabinet and wear sterile gown, close the zipper up to neck and put a head guard.
5.1.4 Check that the gown is properly set or not in a mirror
5.1.5 Wear the booties and tuck the gown in the booties and tie it as required and Cross the Changeover bench.
5.1.6 Enter in to secondary change room by pushing the door. Disinfectant the hands with a disinfectant solution provided.
5.1.7 Enter into the Laminar Airflow area by pushing the door.
5.1.8 Unloaded the media with the help of Asbestos gloves from the autoclave xxxxx controlled area door and arrange them on the table provided under Vertical flow ceiling suspended laminar.
5.1.9 Remove the gloves and wear sterile gloves and disinfectant them by disinfectant solution before start of work.
5.2 Exit procedure
5.2.1 Once the testing in Bio safety cabinet is completed arrange all the items in stainless steel trays and pass it through the pass box provided in the Change room –I.
5.2.2 Clean the Bio safety cabinet as per current version of Sop No xxxxx
5.2.3 Remove the gloves and put them in dustbin.
5.2.4 After cleaning Proceed for Exit.
5.2.5 Enter in to the change room –II by pulling the door.
5.2.6 Remove the sterile garments, Booties and put them in soiled garment box provided in the change room -II.
5.2.7 Enter in to the Change room I by pulling the door.
5.2.8 Cross the Changeover bench.
5.2.9 Wear the apron, cap and foot ware kept in the designated racks.
5.2.10 Enter in to the Air lock room by pulling the door.
END OF DOCUMENT
Gowning, procedure, controlled, microbiology
