To lay down the procedure for media stock maintenance and suitability testing of media containers.
1.0 OBJECTIVE:
To lay down the procedure for media stock maintenance and suitability testing of media containers.
2.0 SCOPE:
This procedure is applicable for media used in microbiology laboratory.
• Fresh lot of media containers
• Media containers, which are periodically used & stored for prolonged period.
3.0 RESPONSIBILITY :
Microbiologist. : To perform the procedure as per SOP
Head – Quality Control: To ensure the compliance of the SOP.
4.0 PROCEDURE :
4.1 Media Stock Maintenance:
4.1.1 Receive the media from the supplier. Check the bottles/ boxes of media for the manufacturing date and expiry date.
4.1.2 Incase the expiry date is within one year (except for the solutions where the expiry date labeled itself is one year or less), reject the same and send back to the supplier.
4.1.3. Paste the label for the media bottle / box.
4.1.4 For each new batch of dehydrated media received, carry out the growth promotion test of the media as per Growth Promotion Test before using for Microbiological analysis.
4.1.5 If media complies then release the lot, if not complies reject the lot.
4.1.6 Store dehydrated media in tightly closed packs in the dark or as directed by the manufacturer.
4.1.7 Enter the details of the receipt of the media in the Media Stock Record.
4.1.8 Enter the details in such a way that incase there are 2 boxes/ bottles of media of the same batch 2 lines are allotted for the further record of the same.
4.1.9 The next 2-bottles/ boxes with a different batch number will be entered after that and 2 lines will be allotted for that batch.
4.1.10 Ensure that the expiry date of the bottles are in chronological order if more than one batch is received.
4.1.11 Incase there are smaller packets in a box each individual pack shall be labeled.
4.1.12 Ensure that the media stock is used on a First In First Out or First Expiry First Out basis and that only one pack of medium is in use at any given time.
4.1.13 On opening a new pack, enter the date of opening on the label of the bottle/ box and in the media stock register.
4.1.14 Follow the media preparation instructions on the label for rehydration.
4.2 Retest of the media bottles:
4.2.1 Check that the media is having any lumps formation, if any discard the media.
NOTE : Media containers, which fail for suitability, shall not be used for testing.
Microbiology, rehydration, procedure
