To lay down the procedure for operation and calibration of micropipette.
1.0 OBJECTIVE:
To lay down the procedure for operation and calibration of micropipette.
20 SCOPE:
This SOP is applicable to operation and calibration of micropipette in Microbiology department.
3.0 RESPONSIBILITY:
QC – Microbiologist
Head – Quality Control
4.0 PROCEDURE:
4.1 Operation :
4.1.1 Hold the micropipette in such a way that griper should rest on your pointer finger.
4.1.2 Put your thumb on operation button.
4.1.3 Fix the micro tip-to-tip cone.
4.1.4 Adjust the volume of solution as required by rotating operation button..
4.1.5 Press the operation button.
4.1.6 Dip the micro tip not less than 1 cm below the surface of solution.
4.1.7 Release the operation button now to fill the required volume of liquid.
4.1.8 Hold the micropipette above the point where solution is to be discharged.
4.1.9 Again press the operation button to discharge the liquid.
4.1.10 After the use of micropipette press the ejector to remove the micro tip.
4.2 Precaution
4.2.1 Do not press operation button very hard.
4.2.2 Handle the pipette carefully.
4.2.3 Fix the micro tip to tip cone.
4.2.4 Eject the microtip with help of “Ejector and Pusher” only.
4.3 Calibration:
4.3.1 Use calibrated balance.
4.3.2 Put empty beaker on the balance.
4.3.3 Tare the weight.
4.3.4 Take known volume of water in the microtip of
4.3.6 Note the weight of purified water.
4.3.7 Repeat the above operation for further 4 times.
4.3.8 Record the weight.
4.3.9 Specific gravity of water is 0.99602 gm/ml.
4.3.10 Calculation :
Weight in gms = volume of water x specific gravity.
4.3.11 Limit: ± 0. 001gm
4.3.12 Frequency: Once in a year.
4.3.13 Out of calibration
If micropipette is out of calibration, affix “UNDER MAINTENANCE “ or “DO NOT USE” label on the instrument and immediately service engineer.
Micropipette, microbiology, operation, microbiology
