To lay down the procedure for Operation, Cleaning and Calibration of Bacteriological Incubator
1.0 PURPOSE
1.1 To lay down the procedure for Operation, Cleaning and Calibration of Bacteriological Incubator (Thermolab) Equipment identification Nos: XXXXX & XXXXX
2.0 SCOPE
2.1 This Sop covers the Operating, Cleaning and Calibration procedure of Bacteriological incubators.
3.0 RESPONSIBILITY
3.1 Microbiologist
4.0 ACCOUNTABILITY
4.1 Head - Quality Control
5.0 PROCEDURE
5.1 Operation
5.1.1 Connect power plug to the socket.
5.1.2 Put ON the Mains on the control panel, Orange lamp inside it glows and temperature controller as well as the PC / Interface displays and the inside Incubator motor ON.
5.1.3 Put ON the heating switch on the control panel, Dry heater will ON and green indicator lamp glows.
5.1.4 Set the required temperature on the temperature controller by pressing increment and decrement key, which will display the Set point.
5.1.5 Set the safety thermostat at 5° above the Set temperature on the temperature controller.
5.1.6 The safety thermostat will cut off the Mains contractor in case of over shoot of the temperature by 5°C with a beep sound alarm.
5.1.7 Set the temperature by pressing up or down key and decrement key.
5.1.8 Now the bottom display (green digital) will show the set temperature and the top display (Red digital) will show the actual temperature.
5.1.9 The PC / Printer Interface shows both the Set temperature and Actual temperature.
5.1.10 Set the Print time interval on PC / Printer Interface unit and connect the same to 80 column printer or to the PC for data communication.
5.1.11 Switch ON the Fluorescent Light switch when necessary.
5.1.12 Enter the temperature in to the temperature Monitoring record as per annexure - I.
5.1.13 Enter the Incubated sample details in Incubator log record as per annexure –II
? Keep the incubator in a room having constant temperature and away from any heating device
? Record the temperature details two times a day in the morning and in the evening respectively.
5.2 CLEANING
5.2.1 Put ‘OFF’ the Heating, MAINS switch and disconnect the power supply respectively.
5.2.2 Empty the Bacteriological incubator by removing all the materials present inside it.
5.2.3 Clean the inner walls and trays using wet mop followed by spraying 70% Iso Propyl Alcohol and allow it to dry
5.2.4 Ensure that the incubator is dry.
5.2.5 Replace all the materials taken from it, and switch “ON” the Bacteriological incubator.
5.2.6 Prepare the Cleaning solution as per the current version of disinfectant solution preparation Sop No – XXXXX
5.2.7 After cleaning of the incubator enter the details in Incubator cleaning record as per Annexure–III
Frequency: Once in a week / or during any spillage
5.3 Calibration
5.3.1 Switch off the instrument and unload all the materials and shift to another Incubator by setting the same temperature of the unloaded incubator.
5.3.2 Remove the cork from the validation porthole and insert the thermal probes.
5.3.3 Fix the thermal probes as per schematic diagram shown in the Annexure -IV.
5.3.4 Close the Cork of the porthole.
5.3.5 Switch on the instrument after setting the temperature probes inside the incubator.
5.3.6 Once the temperature is attained to the set temperature start the data logger.
5.3.7 Record the data logger and the display temperature for every 15 minutes.
5.3.8 Monitor the temperature for 2 hours of each set temperature.
5.3.9 Variation between the probes temperature and the display temperature should be ? 2°C.
Frequency: Once in a Year and if any maintenance activity is done.
END OF DOCUMENT
Procedure, cleaning, bacteriological, incubators, microbiology
