sampling of Water

1.0    OBJECTIVE: 

To lay down a procedure for Sampling of Water from different sampling points of Water system for Chemical & Microbiological analysis.

2.0    SCOPE:  

This SOP is applicable for sampling of Potable Water (Raw water),Treated water and Purified water from designated sampling points.

3.0    RESPONSIBILITY: 

Microbiologist : To perform the procedure as per SOP

Head –Quality Control : To ensure the compliance of SOP.

4.0    PROCEDURE:

4.1    Preparation of Sampling Bottles:

4.1.1    Cleaned and dried glassware shall be used for sampling of water.

4.1.2    For Microbiological analysis, sterilized Sampling Bottles should be used and for Potable water (Raw water) and for reated Water   add 5ml of 5 percent Sodium Thiosulphate into sampling bottles before sterilization to neutralize the chlorine in water samples.

4.1.3    All Bottles used for sampling shall be properly stoppered & kept in Poly bag before taking it to the sampling points.

4.2    Sampling Procedure for Microbiological Analysis

4.2.1    Wear gloves, masks and wipe hands with 70%v/v IPA.

4.2.2    Spray the exterior of sampling point with 70% IPA and leave it for a contact period of 1-2 minutes.

4.2.3    Take the sterile sampling bottles required for sampling to the respective sampling point in closed condition.

4.2.4    Open the lid of Bottle carefully to avoid any contamination from atmosphere, collect the sample without rinsing and close the bottle immediately.

4.2.5    Label the Bottles with details of Sampling point, date of sampling and transfer the bottles carefully to Microbiology Laboratory. 

4.3    Sampling Procedure for Chemical Analysis

4.3.1 For Chemical analysis (PH and Conductivity) of Purified water, cleaned and dried Bottles of 250 ml capacity to be used and Hcl washed bottles of 250ml capacity to be used for TOC analysis.

4.1.2Rinse the flask twice with water, then collect the sample for chemical analysis.

4.3.3    Dried and cleaned Bottles of 500 ml capacity to be used for chemical testing of Potable water (Raw water) and Treated Water.

4.3.4    Label the Bottles with details of Sampling point and date of sampling. 

4.4    Precautions during sampling: 

4.4.1    Wear gloves and nose masks before collecting sample.

4.4.2    During sampling, the flow from the sampling point shall be restricted to permit filling of the Bottles without splashing.

4.4.3  During sampling remove the stopper by taking all care to prevent any external contamination.

4.4.4    Those sampling points that are connected with hose line the samples should be drawn at the end of the hose line.

4.4.5  After sampling carefully transfer the samples to Microbiology laboratory and enter in inward register.

4.4.6    Test water samples as per STPs of Purified Water and Treated water, Potable Water (Raw water) 

Record the results in the respective analysis worksheet.

4.5    Testing For Sampling Point: The sampling points and their locations 

4.6    The quantity of water to be sampled for chemical as well as microbiological is given in following tables.

4.7 AR Numbering system for water samples

For Purified water

YYPWNNN

YY – Year code like 16,17…

PW – Purified Water

NNN – Sequential Number like 001,002,003……

Note : At every year starting sequential number starts from 001.

Example: 16PW001 indicates A.R No. of Purified water for the first sample of the year 2016 and for second sample A.R.No is 16PW002,……

Each sample should be identified by a separate A.R.No

For Potable water (Raw Water)

YYRWNNN

YY – Year code like 16,17…

RW – Raw Water 

NNN – Sequential Number like 001,002,003……

Note : At every year starting sequential number starts from 001.

Example: 16RW001 indicates A.R No. of Purified water for the first sample of the year 2016 and for second sample A.R.No is 16RW002,……

Each sample should be identified by a separate A.R.No

For Treated Water

YYTWNNN

YY – Year code like 16,17…

TW – Treated Water

NNN – Sequential Number like 001,002,003……

Note : At every year starting sequential number starts from 001.

Example: 16TW001 indicates A.R No. of Purified water for the first sample of the year 2016 and for second sample A.R.No is 16TW002,……

Each sample should be identified by a separate A.R.No

4.8    Enter the sampling details and analysis 

4.9    Once in a year after monsoon send GIDC water to outside lab for complete analysis Sampling Schedule 

Microbiology, hcl, water