1.0 PURPOSE
1.1 To lay down the procedure for disposal of microbial cultures and culture media.
2.0 SCOPE:
2.1 This SOP covers the procedure for disposal of microbial cultures and culture media in the Microbiology Laboratory.
3.0 RESPONSIBILITY:
3.1 Microbiologist.
4.0 ACCOUNTABILITY:
4.1 Head – Quality Control
5.1 PROCEDURE:
5.1 Upon observation, collect used media flasks, bottles, petri dishes and cultures slants/suspensions in Stainless Steel box.
5.2 Load the box in the single door autoclave and sterilize.
5.3 Collect destructed liquid and solid media in the Stainless steel container and send to Effluent treatment plant.
5.4 Clean all the glassware after discarding the media.
5.5 After washing pour 500 mL of 2.5% Dettol or Savlon solution in the washing sink, to disinfect the drain line.
5.6 Record the details in Destruction record for culture media as per QCxxxxx
END OF DOCUMENT
Microbiology, microbial cultures, culture media