To lay down the procedure for identification of the bacterial colonies obtained during the plate exposure in production and microbiology area to know about the flora present in the controlled environment.
1.0 PURPOSE
1.1 To lay down the procedure for identification of the bacterial colonies obtained during the plate exposure in production and microbiology area to know about the flora present in the controlled environment.
2.0 SCOPE
2.1 This SOP covers the procedure for identification of bacterial isolates in the exposed plates
3.0 RESPONSIBILITY
3.1 Microbiologist
4.0 ACCOUNTABILITY
4.1 Department head- Quality Control
5.0 PROCEDURE
5.1 Pick the individual colonies from the previously exposed plates in which growth found.
5.2 Purify the colonies, if necessary by streaking on to Soyabean casein digest agar plate.
5.3 Observe and record the morphological characters of the colonies such as shape, elevation, color and margins in Bacterial isolate identification as per annexure-I.
5.4 Using sterile inoculating technique, inoculate the organism on two Soyabean casein digest agar slants. Use one slant culture to determine the cultural characteristics and the other to repeat any of the tests, if necessary as stock.
5.5 Make a smear of the colony by taking with a loop and subject it to Gram’s Staining procedure. Observe under microscope and note the reaction, morphology andarrangement of the cells.
5.6 Using aseptic technique, inoculate the required media, to carry out the biochemical tests as given in Annexure-I (B) and incubate at 35°C for 24 to 72 hrs. Record the results.
END OF DOCUMENT
Procedur, identification, bacterial isolate , microbiology
